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rnase h  (New England Biolabs)


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    New England Biolabs rnase h
    Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m0297/RNase+H/pmc12926610-373-32-34
    Average 99 stars, based on 5390 article reviews
    rnase h - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    DNA Synthesis:

    Article Title: Glutathione S-transferase Theta 2 causes drug resistance by inhibiting arsenic trioxide-induced ferroptosis in pancreatic ductal adenocarcinoma.
    Article Snippet: 27 Chemotherapy remains the cornerstone for the treatment of pancreatic ductal 28 adenocarcinoma (PDAC).. However, their efficacy is often undermined by the 29 emergence of drug resistance.. Recent evidence has highlighted the critical role of 30 ferroptosis inhibition in mediating this resistance.

    other:

    Article Title: A High-Throughput Screening Pipeline to Identify Methyltransferase and Exonuclease Inhibitors of SARS-CoV-2 NSP14.
    Article Snippet: SARS-CoV-2 infections led to a worldwide pandemic in 2020.. As of 2024, therapeutics against SARS-CoV-2 have continued to be desirable.. NSP14 is a dual-function methyltransferase (MTase) and exonuclease (ExoN) with key roles in SARS-CoV-2 genome propagation and host immune system evasion.

    Concentration Assay:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    CRISPR:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Recombinase Polymerase Amplification:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Polymerase Chain Reaction:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Extraction:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Lysis:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Lyophilization:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Agarose Gel Electrophoresis:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Marker:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Virus:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Sampling:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Sterility:

    Article Title: Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device
    Article Snippet: .. Stock Concentration LwaCas13a Single-step CRISPR Tolo Biotech Co., Ltd 32117-01 10 μM RPA lyophilized pellets Single-step CRISPR TwistDxTM TABAS03KIT / Rehydration buffer Single-step CRISPR TwistDxTM TABAS03KIT / T7 RNA polymerase Single-step CRISPR New England Biolabs® Inc. M0251 50 U/μL RNase H Single-step CRISPR New England Biolabs® Inc. M0297 5 U/μL RNase Inhibitor Single-step CRISPR Solarbio® Life Science R8061 40 U/μL rNTP mix Single-step CRISPR New England Biolabs® Inc. N0466 25 mM Monkeypox pseudovirus Single-step CRISPR and PCR Fubio FNDV4731 / DNase/RNase- free water / Solarbio® Life Science R1600 / HiScribe TM T7 High Yield RNA Synthesis Kit IVT New England Biolabs® Inc. E2040 / Standard Taq buffer IVT New England Biolabs® Inc. B9014 10× RNA Clean & Concentrator-5 Kit IVT ZYMO RESEARCH R1013 / RQ1 RNase- Free DNase IVT Promega M6101 10 U/μL QubitTM RNA Broad Range Assay Kits IVT Thermo Fisher ScientficTM Q10211 / Viral Extraction kit Viral extraction Tiangen Biotech DP315-R / Chelex-100 Viral lysis Solarbio® Life Science C8230 / TCEP Viral lysis Beyotime Biotechnolog y ST049 500 mM Tris (pH8.0) Viral lysis Thermo Fisher ScientficTM 15568025 1 M BSA Lyophilization Sigma- Aldrich 10711454001 / Taq buffer qPCR Fapon Biotech MD099M 5× Taq master mix qPCR Fapon Biotech MD099M 25× Loading dye Agarose gel electrophoresis NEB B7021S 6× Agarose Agarose gel electrophoresis Invitrogen 75510019 / DNA marker Agarose gel electrophoresis Tiangen MD108 / Disposable virus sampling tube Sample collection Yocon Biology MT0301 / Disposable sterile sampling cup Sample collection BKMAM Biotechnolog y 110413005 / Supplementary Table 6. ..

    Incubation:

    Article Title: DNA damage induced by HIV-1 Vpr triggers epigenetic remodeling and transcriptional programs to enhance virus transcription and latency reactivation
    Article Snippet: .. RNase H-treated cells were incubated with 150 U/mL of enzyme, or buffer alone, for 2 h at 37° (NEB, M0297). ..



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